Purpose: Cell homeostasis is an essential factor in maintaining cellular health and intracellular ‎mechanisms, which can also be associated with inflammatory factors and increased stress for ‎muscle cell health. Calcium is also a necessary factor in cell health and is to establish ‎homeostasis in the body‏.‏‎ This study aimed to investigate the effect of high-intensity training on ‎serum calcium levels in combat women.‎ Method: For this purpose, 18 women karateka in the age range of 17 to 23 years were randomly divided ‎into 2 groups: control (n=7) and exercise ((n=11). The exercise group performed proper ‎exercises to improve karate and aerobic techniques for 6 weeks in the intensities of more than ‎‎80% of the maximum heart rate reserve.  Physiological indices and blood samples were ‎measured 24 hours before and 48 hours after the training protocol.  To measure calcium cell ‎biomarker, the Pars Azmoon calcium kit was used.  Research data were analysed by covariance ‎test at the significant level (P≤0.05) using SPSS software version 25‎.‎ Results: After six weeks of high-intensity training, there was a significant difference in serum calcium ‎levels in the exercise group compared to the control group (P = 0.002).  Also, the results within ‎the group showed a significant increase in serum calcium (P = 0.016) in the exercise group ‎compared to baseline conditions.‎ Conclusions: High intensity training seems to increase serum calcium in female athletes, and this can lead to ‎precursors to cellular disorders, muscle damage and increase inflammatory factors and ‎ultimately decrease the performance of female athletes. However, more research is essential in ‎this regard‎.‎